• Immunofluorescence analysis of NIH-3T3 cells using UBE2G2 antibody (STJ112443) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using UBE2G2 antibody (STJ112443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-UBE2G2 antibody (1-165) (STJ112443)

SKU:
STJ112443

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBE2G2 (1-165) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2G2
Gene ID: 7327
Uniprot ID: UB2G2_HUMAN
Immunogen Region: 1-165
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human UBE2G2 (NP_003334.2).
Immunogen Sequence: MAGTALKRLMAEYKQLTLNP PEGIVAGPMNEENFFEWEAL IMGPEDTCFEFGVFPAILSF PLDYPLSPPKMRFTCEMFHP NIYPDGRVCISILHAPGDDP MGYESSAERWSPVQSVEKIL LSVVSMLAEPNDESGANVDA SKMWRDDREQFYKIAKQIVQ KSLGL
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD). Required for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation.
Protein Name Ubiquitin-Conjugating Enzyme E2 G2
E2 Ubiquitin-Conjugating Enzyme G2
Ubiquitin Carrier Protein G2
Ubiquitin-Protein Ligase G2
Database Links Reactome: R-HSA-8866652
Reactome: R-HSA-983168
Cellular Localisation Endoplasmic Reticulum
Lipid Droplet
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 G2 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme G2 antibody
Anti-Ubiquitin Carrier Protein G2 antibody
Anti-Ubiquitin-Protein Ligase G2 antibody
Anti-UBE2G2 antibody
Anti-UBC7 antibody

Information sourced from Uniprot.org

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