• Western blot analysis of various lysates using UBE2E2 Rabbit polyclonal antibody (STJ118869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-UBE2E2 antibody (1-201) (STJ118869)

SKU:
STJ118869

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2E2
Gene ID: 7325
Uniprot ID: UB2E2_HUMAN
Immunogen Region: 1-201
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human UBE2E2 (NP_689866.1).
Immunogen Sequence: MSTEAQRVDDSPSTSGGSSD GDQRESVQQEPEREQVQPKK KEGKISSKTAAKLSTSAKRI QKELAEITLDPPPNCSAGPK GDNIYEWRSTILGPPGSVYE GGVFFLDITFSPDYPFKPPK VTFRTRIYHCNINSQGVICL DILKDNWSPALTISKVLLSI CSLLTDCNPADPLVGSIATQ YMTNRAEHDRMARQWTKRYA T
Post Translational Modifications Autoubiquitinated in vitro.
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.
Protein Name Ubiquitin-Conjugating Enzyme E2 E2
E2 Ubiquitin-Conjugating Enzyme E2
Ubch8
Ubiquitin Carrier Protein E2
Ubiquitin-Protein Ligase E2
Database Links Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 E2 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme E2 antibody
Anti-Ubch8 antibody
Anti-Ubiquitin Carrier Protein E2 antibody
Anti-Ubiquitin-Protein Ligase E2 antibody
Anti-UBE2E2 antibody
Anti-UBCH8 antibody

Information sourced from Uniprot.org

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