• Immunohistochemistry analysis of paraffin-embedded human esophagus using UBE2A antibody (STJ110055) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using UBE2A antibody (STJ110055) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using UBE2A antibody (STJ110055) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using UBE2A antibody (STJ110055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-UBE2A antibody (1-152) (STJ110055)

SKU:
STJ110055

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBE2A (1-152) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2A
Gene ID: 7319
Uniprot ID: UBE2A_HUMAN
Immunogen Region: 1-152
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2).
Immunogen Sequence: MSTPARRRLMRDFKRLQEDP PAGVSGAPSENNIMVWNAVI FGPEGTPFEDGTFKLTIEFT EEYPNKPPTVRFVSKMFHPN VYADGSICLDILQNRWSPTY DVSSILTSIQSLLDEPNPNS PANSQAAQLYQENKREYEKR VSAIVEQSWRDC
Post Translational Modifications Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
Protein Name Ubiquitin-Conjugating Enzyme E2 A
E2 Ubiquitin-Conjugating Enzyme A
Rad6 Homolog A
Hr6a
Hhr6a
Ubiquitin Carrier Protein A
Ubiquitin-Protein Ligase A
Database Links Reactome: R-HSA-8866652
Reactome: R-HSA-8866654
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 A antibody
Anti-E2 Ubiquitin-Conjugating Enzyme A antibody
Anti-Rad6 Homolog A antibody
Anti-Hr6a antibody
Anti-Hhr6a antibody
Anti-Ubiquitin Carrier Protein A antibody
Anti-Ubiquitin-Protein Ligase A antibody
Anti-UBE2A antibody
Anti-RAD6A antibody

Information sourced from Uniprot.org

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