• Western blot analysis of lysates from HEL cells, using Ube1L/UBA7 Rabbit monoclonal antibody (STJ11105161) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-UBA7 antibody (1-100) [S5161RM] (STJ11105161)

SKU:
STJ11105161

€183.13 - €799.38
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ubiquitin-Like Modifier-Activating Enzyme 7 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5161RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBA7
Gene ID: 7318
Uniprot ID: UBA7_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Ube1L/UBA7 (NP_003326.2).
Immunogen Sequence: MDALDASKLLDEELYSRQLY VLGSPAMQRIQGARVLVSGL QGLGAEVAKNLVLMGVGSLT LHDPHPTCWSDLAAQFLLSE QDLERSRAEASQELLAQLNR
Tissue Specificity Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
Post Translational Modifications ISGylated.
Function Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.
Protein Name Ubiquitin-Like Modifier-Activating Enzyme 7
Ubiquitin-Activating Enzyme 7
D8
Ubiquitin-Activating Enzyme E1 Homolog
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-168928
Reactome: R-HSA-5656169
Reactome: R-HSA-936440
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Like Modifier-Activating Enzyme 7 antibody
Anti-Ubiquitin-Activating Enzyme 7 antibody
Anti-D8 antibody
Anti-Ubiquitin-Activating Enzyme E1 Homolog antibody
Anti-UBA7 antibody
Anti-UBE1L antibody
Anti-UBE2 antibody

Information sourced from Uniprot.org

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