| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Ubiquitin-like modifier-activating enzyme 5 (281-330 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBA5 |
| Gene ID: | 79876 |
| Uniprot ID: | UBA5_HUMAN |
| Immunogen Region: | 281-330 aa |
| Specificity: | Uba5 Polyclonal Antibody detects endogenous levels of Uba5 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human UBA5 at the amino acid range 281-330 |
| Function | E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme 5 Ubiquitin-Activating Enzyme 5 Thifp1 Ufm1-Activating Enzyme Ubiquitin-Activating Enzyme E1 Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm Nucleus Endoplasmic Reticulum Membrane Golgi Apparatus Localizes Mainly In The Cytoplasm While It Localizes To The Nucleus In Presence Of Sumo2 Interaction With Gabarapl2 Promotes Localization To The Endoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-Ubiquitin-Like Modifier-Activating Enzyme 5 antibody Anti-Ubiquitin-Activating Enzyme 5 antibody Anti-Thifp1 antibody Anti-Ufm1-Activating Enzyme antibody Anti-Ubiquitin-Activating Enzyme E1 Domain-Containing Protein 1 antibody Anti-UBA5 antibody Anti-UBE1DC1 antibody |
Information sourced from Uniprot.org