| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-UBA5 (1-404) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UBA5 |
| Gene ID: |
79876 |
| Uniprot ID: |
UBA5_HUMAN |
| Immunogen Region: |
1-404 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human UBA5 (NP_079094.1). |
| Immunogen Sequence: |
MAESVERLQQRVQELERELA QERSLQVPRSGDGGGGRVRI EKMSSEVVDSNPYSRLMALK RMGIVSDYEKIRTFAVAIVG VGGVGSVTAEMLTRCGIGKL LLFDYDKVELANMNRLFFQP HQAGLSKVQAAEHTLRNINP DVLFEVHNYNITTVENFQHF MDRISNGGLEEGKPVDLVLS CVDNFEARMTINTACNELGQ TWMESGVSENAVSGHIQLII PGESACFACAPPLVVAANI |
| Tissue Specificity | Widely expressed. |
| Function | E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress. Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme 5Ubiquitin-Activating Enzyme 5Thifp1Ufm1-Activating EnzymeUbiquitin-Activating Enzyme E1 Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusEndoplasmic Reticulum MembraneGolgi ApparatusLocalizes Mainly In The CytoplasmWhile It Localizes To The Nucleus In Presence Of Sumo2Interaction With Gabarapl2 Promotes Localization To The Endoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-Ubiquitin-Like Modifier-Activating Enzyme 5 antibodyAnti-Ubiquitin-Activating Enzyme 5 antibodyAnti-Thifp1 antibodyAnti-Ufm1-Activating Enzyme antibodyAnti-Ubiquitin-Activating Enzyme E1 Domain-Containing Protein 1 antibodyAnti-UBA5 antibodyAnti-UBE1DC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/100335/386658/STJ25123_1__59754.1713155779.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
