| Short Description : | Rabbit polyclonal anti-NEDD8-activating enzyme E1 catalytic subunit (105-155 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-2000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBA3 |
| Gene ID: | 9039 |
| Uniprot ID: | UBA3_HUMAN |
| Immunogen: | Synthesized peptide derived from the human UBA3 at the amino acid range 105-155 |
| Immunogen Region: | 105-155 aa |
| Specificity: | This antibody detects endogenous levels of UBA3 at Human/Mouse/Rat |
| Function | Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. |
| Protein Name | Nedd8-Activating Enzyme E1 Catalytic SubunitNedd8-Activating Enzyme E1cUbiquitin-Activating Enzyme E1cUbiquitin-Like Modifier-Activating Enzyme 3Ubiquitin-Activating Enzyme 3 |
| Database Links | Reactome: R-HSA-5607761Reactome: R-HSA-5676590Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nedd8-Activating Enzyme E1 Catalytic Subunit antibodyAnti-Nedd8-Activating Enzyme E1c antibodyAnti-Ubiquitin-Activating Enzyme E1c antibodyAnti-Ubiquitin-Like Modifier-Activating Enzyme 3 antibodyAnti-Ubiquitin-Activating Enzyme 3 antibodyAnti-UBA3 antibodyAnti-UBE1C antibody |
Information sourced from Uniprot.org
