• Western blot analysis of extracts of various cell lines, using UAP1 rabbit monoclonal antibody (STJ11102347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U-2 OS cells using UAP1 Rabbit monoclonal antibody (STJ11102347) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using UAP1 rabbit monoclonal antibody (STJ11102347) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using UAP1 Rabbit monoclonal antibody (STJ11102347) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using UAP1 rabbit monoclonal antibody (STJ11102347) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using UAP1 Rabbit monoclonal antibody (STJ11102347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-UAP1 antibody (400-500) [S7MR] (STJ11102347)

SKU:
STJ11102347

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-UAP1 (400-500) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UAP1
Gene ID: 6675
Uniprot ID: UAP1_HUMAN
Immunogen Region: 400-500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human UAP1 (Q16222).
Immunogen Sequence: EDEFSPLKNADSQNGKDNPT TARHALMSLHHCWVLNAGGH FIDENGSRLPAIPRSATNGK SETITADVNHNLKDANDVPI QCEISPLISYAGEGLESYVA D
Tissue Specificity Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver.
Function Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.
Protein Name Udp-N-Acetylhexosamine Pyrophosphorylase
Antigen X
Agx
Sperm-Associated Antigen 2 Includes - Udp-N-Acetylgalactosamine Pyrophosphorylase
Agx-1 - Udp-N-Acetylglucosamine Pyrophosphorylase
Agx-2
Database Links Reactome: R-HSA-446210
Cellular Localisation Cytoplasm
In Spermatozoa
Localized To The Principal Piece Of The Tail
The Neck Region Of The Head And To A Lesser Extent
The Midpiece Of The Tail
Alternative Antibody Names Anti-Udp-N-Acetylhexosamine Pyrophosphorylase antibody
Anti-Antigen X antibody
Anti-Agx antibody
Anti-Sperm-Associated Antigen 2 Includes - Udp-N-Acetylgalactosamine Pyrophosphorylase antibody
Anti-Agx-1 - Udp-N-Acetylglucosamine Pyrophosphorylase antibody
Anti-Agx-2 antibody
Anti-UAP1 antibody
Anti-SPAG2 antibody

Information sourced from Uniprot.org

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