Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Type S APL using Type S APL Rabbit polyclonal antibody (STJ11105607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 40. 5  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Type S APL antibody (388-487) (STJ11105607)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105607
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General Information

Short DescriptionRabbit polyclonal Type S APL (388-487) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPML
RARA
Gene ID5371
5914
Uniprot IDPML_HUMAN
RARA_HUMAN
Immunogen Region388-487
Immunogen SequenceSCITQGKDAAVSKKASPEAA STPRDPIDVDLPEEAERVKA QVQALGLAEAQPMAVVQSVP GAHPVPVYAFSIKGPSYGED VSNTTTAQKRKCSQTQCPRK MASNSSSCPTPGGGHLNGYP VPPYAFFFPPMLGGLSPPGA LTTLQHQLPVSGYSTPSPAT IETQSSSSEEIVPSPPSPPP LPRIYKPCFVCQDKSSGYHY
SpecificityA synthetic peptide corresponding to a sequence within amino acids 388-487AA + 1-100AA of human Type S APL (NP_150241.2/NP_000955.1).

Additional Info

Tissue Specificity P29590 - NA
P10276 - Expressed in monocytes.
Alternative Antibody Names Anti-Protein Pml antibody
Anti-E3 Sumo-Protein Ligase Pml antibody
Anti-Promyelocytic Leukemia Protein antibody
Anti-Ring Finger Protein 71 antibody
Anti-Ring-Type E3 Sumo Transferase Pml antibody
Anti-Tripartite Motif-Containing Protein 19 antibody
Anti-Trim19 antibody
Anti-PML antibody
Anti-MYL antibody
Anti-PP8675 antibody
Anti-RNF71 antibody
Anti-TRIM19 antibody
Anti-Retinoic Acid Receptor Alpha antibody
Anti-Rar-Alpha antibody
Anti-Nuclear Receptor Subfamily 1 Group B Member 1 antibody
Anti-RARA antibody
Anti-NR1B1 antibody

Information sourced from Uniprot.org

Citations

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