![Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89709/358191/STJ11100773_1__48709.1713122506.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89709/358191/STJ11100773_1__48709.1713122506.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TWF2 knockout (KO) 293T cells, using [KO Validated] TWF2 Rabbit polyclonal antibody (STJ11100773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TWF2 |
| Gene ID: | 11344 |
| Uniprot ID: | TWF2_HUMAN |
| Immunogen Region: | 1-369 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1). |
| Immunogen Sequence: | MAHQTGIHATEELKEFFAKA RAGSVRLIKVVIEDEQLVLG ASQEPVGRWDQDYDRAVLPL LDAQQPCYLLYRLDSQNAQG FEWLFLAWSPDNSPVRLKML YAATRATVKKEFGGGHIKDE LFGTVKDDLSFAGYQKHLSS CAAPAPLTSAERELQQIRIN EVKTEISVESKHQTLQGLAF PLQPEAQRALQQLKQKMVNY IQMKLDLERETIELVHTEPT DVAQLPSRVPRDAARYHFF |
| Tissue Specificity | Ubiquitously expressed (at protein level). |
| Post Translational Modifications | In vitro, phosphorylated by PRKCZ, CK2 and SRC. |
| Function | Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia. |
| Protein Name | Twinfilin-2 A6-Related Protein Ha6rp Protein Tyrosine Kinase 9-Like Twinfilin-1-Like Protein |
| Database Links | Reactome: R-HSA-9662360 Reactome: R-HSA-9662361 |
| Cellular Localisation | Cytoplasm Cytoskeleton Perinuclear Region Cell Projection Stereocilium Perinuclear And G-Actin-Rich Cortical Actin Structure Sublocalization |
| Alternative Antibody Names | Anti-Twinfilin-2 antibody Anti-A6-Related Protein antibody Anti-Ha6rp antibody Anti-Protein Tyrosine Kinase 9-Like antibody Anti-Twinfilin-1-Like Protein antibody Anti-TWF2 antibody Anti-PTK9L antibody Anti-MSTP011 antibody |
Information sourced from Uniprot.org