| Host: |
Mouse |
| Applications: |
WB/IHC/ICC |
| Reactivity: |
Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Human/Non-Human Primates/Rabbit/Sheep/Vole |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Tubulin beta III is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
AA10 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Purification: |
This antibody was protein g purified culture from supernatant. |
| Dilution Range: |
WB 1:10000IHC 1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Tubb3 |
| Gene ID: |
246118 |
| Uniprot ID: |
TBB3_RAT |
| Immunogen: |
Synthetic peptide corresponding to amino acid residues specific to beta III tubulin conjugated to KLH. |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglycylated, resulting in polyglycine chains on the gamma-carboxyl group. Glycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering polyglycylation levels increases polyglutamylation, and reciprocally. Cilia and flagella glycylation is required for their stability and maintenance. Flagella glycylation controls sperm motility. Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. |
| Protein Name | Tubulin Beta-3 ChainNeuron-Specific Class Iii Beta-Tubulin |
| Database Links | Reactome: R-RNO-190840Reactome: -RNO-2132295Reactome: -RNO-2467813Reactome: -RNO-2500257Reactome: -RNO-3371497Reactome: -RNO-380320Reactome: -RNO-437239Reactome: -RNO-5610787Reactome: -RNO-5617833Reactome: -RNO-5620924Reactome: -RNO-5626467Reactome: -RNO-5663220Reactome: -RNO-6807878Reactome: -RNO-6811434Reactome: -RNO-6811436Reactome: -RNO-68877Reactome: -RNO-8852276Reactome: -RNO-8955332Reactome: -RNO-9646399Reactome: -RNO-9648025Reactome: -RNO-9668328Reactome: -RNO-983189Reactome: -RNO-9833482 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionGrowth ConeLamellipodiumFilopodium |
| Alternative Antibody Names | Anti-Tubulin Beta-3 Chain antibodyAnti-Neuron-Specific Class Iii Beta-Tubulin antibodyAnti-Tubb3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
