| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Beta III-Tubulin (351-450) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S7MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TUBB3 |
| Gene ID: |
10381 |
| Uniprot ID: |
TBB3_HUMAN |
| Immunogen Region: |
351-450 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 351-450 of human Beta III-Tubulin (Q13509). |
| Immunogen Sequence: |
VAVCDIPPRGLKMSSTFIGN STAIQELFKRISEQFTAMFR RKAFLHWYTGEGMDEMEFTE AESNMNDLVSEYQQYQDATA EEEGEMYEDDEEESEAQGPK |
| Tissue Specificity | Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. |
| Protein Name | Tubulin Beta-3 ChainTubulin Beta-4 ChainTubulin Beta-Iii |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-190840Reactome: R-HSA-190861Reactome: R-HSA-2132295Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-3371497Reactome: R-HSA-380320Reactome: R-HSA-389957Reactome: R-HSA-389960Reactome: R-HSA-389977Reactome: R-HSA-437239Reactome: R-HSA-5610787Reactome: R-HSA-5617833Reactome: R-HSA-5620924Reactome: R-HSA-5626467Reactome: R-HSA-5663220Reactome: R-HSA-6807878Reactome: R-HSA-6811434Reactome: R-HSA-6811436Reactome: R-HSA-68877Reactome: R-HSA-8852276Reactome: R-HSA-8955332Reactome: R-HSA-9609690Reactome: R-HSA-9609736Reactome: R-HSA-9619483Reactome: R-HSA-9646399Reactome: R-HSA-9648025Reactome: R-HSA-9668328Reactome: R-HSA-983189 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionGrowth ConeLamellipodiumFilopodium |
| Alternative Antibody Names | Anti-Tubulin Beta-3 Chain antibodyAnti-Tubulin Beta-4 Chain antibodyAnti-Tubulin Beta-Iii antibodyAnti-TUBB3 antibodyAnti-TUBB4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
