• Western blot analysis of extracts of various cell lines, using TSPAN4/NAG-2 antibody (STJ112291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Western blot analysis of PC-3, using TSPAN4/NAG-2 rabbit polyclonal antibody (STJ112291) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TSPAN4 antibody (100-238) (STJ112291)

SKU:
STJ112291

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TSPAN4 (100-238) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSPAN4
Gene ID: 7106
Uniprot ID: TSN4_HUMAN
Immunogen Region: 100-238
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-238 of human TSPAN4/NAG-2 (NP_003262.1).
Immunogen Sequence: IAILFFAYTDKIDRYAQQDL KKGLHLYGTQGNVGLTNAWS IIQTDFRCCGVSNYTDWFEV YNATRVPDSCCLEFSESCGL HAPGTWWKAPCYETVKVWLQ E
Tissue Specificity Expressed in multiple tissues but is absent in brain, lymphoid cells, and platelets.
Protein Name Tetraspanin-4
Tspan-4
Novel Antigen 2
Nag-2
Transmembrane 4 Superfamily Member 7
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Tetraspanin-4 antibody
Anti-Tspan-4 antibody
Anti-Novel Antigen 2 antibody
Anti-Nag-2 antibody
Anti-Transmembrane 4 Superfamily Member 7 antibody
Anti-TSPAN4 antibody
Anti-NAG2 antibody
Anti-TM4SF7 antibody

Information sourced from Uniprot.org

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