• Western blot analysis of lysates from COS7 cells, using TS101 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of Hela cell. 1, Tsg 101 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-TSG101 antibody (281-330 aa) (STJ96125)

SKU:
STJ96125

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tumor susceptibility gene 101 protein (281-330 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSG101
Gene ID: 7251
Uniprot ID: TS101_HUMAN
Immunogen Region: 281-330 aa
Specificity: Tsg 101 Polyclonal Antibody detects endogenous levels of Tsg 101 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human TSG101 at the amino acid range 281-330
Post Translational Modifications Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis.the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-ST-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan. It may also play a role in the extracellular release of microvesicles that differ from the exosomes.
Protein Name Tumor Susceptibility Gene 101 Protein
Escrt-I Complex Subunit Tsg101
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-174490
Reactome: R-HSA-917729
Reactome: R-HSA-9610379
Reactome: R-HSA-9615710
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Late Endosome Membrane
Cytoskeleton
Microtubule Organizing Center
Centrosome
Midbody
Midbody Ring
Nucleus
Mainly Cytoplasmic
Membrane-Associated When Active And Soluble When Inactive
Nuclear Localization Is Cell Cycle-Dependent
Interaction With Cep55 Is Required For Localization To The Midbody During Cytokinesis
Alternative Antibody Names Anti-Tumor Susceptibility Gene 101 Protein antibody
Anti-Escrt-I Complex Subunit Tsg101 antibody
Anti-TSG101 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance