| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TSG101 (280-330 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Tsg101 |
| Gene ID: |
22088 |
| Uniprot ID: |
TS101_MOUSE |
| Immunogen Region: |
280-330 aa |
| Specificity: |
Specific for TSG101. |
| Immunogen: |
A synthetic peptide from aa region 280-330 of rat TSG101 conjugated to an immunogenic carrier protein was used as the antigen. |
| Post Translational Modifications | Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1. |
| Function | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis.the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan. It may also play a role in the extracellular release of microvesicles that differ from the exosomes. |
| Protein Name | Tumor Susceptibility Gene 101 ProteinEscrt-I Complex Subunit Tsg101 |
| Database Links | Reactome: R-MMU-917729 |
| Cellular Localisation | CytoplasmEarly Endosome MembranePeripheral Membrane ProteinCytoplasmic SideLate Endosome MembraneCytoskeletonMicrotubule Organizing CenterCentrosomeMidbodyMidbody RingNucleusMainly CytoplasmicMembrane-Associated When Active And Soluble When InactiveNuclear Localization Is Cell Cycle-DependentInteraction With Cep55 Is Required For Localization To The Midbody During Cytokinesis |
| Alternative Antibody Names | Anti-Tumor Susceptibility Gene 101 Protein antibodyAnti-Escrt-I Complex Subunit Tsg101 antibodyAnti-Tsg101 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
