| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TSC22D3 (58-134) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TSC22D3 |
| Gene ID: |
1831 |
| Uniprot ID: |
T22D3_HUMAN |
| Immunogen Region: |
58-134 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 58-134 of human TSC22D3 (NP_004080.2). |
| Immunogen Sequence: |
MDLVKNHLMYAVREEVEILK EQIRELVEKNSQLERENTLL KTLASPEQLEKFQSCLSPEE PAPESPQVPEAPGGSAV |
| Tissue Specificity | Ubiquitously expressed, including in the fetal brain and liver. Expressed in brain, lung, spleen and skeletal muscle. Lower levels detected in heart and kidney. Not detected in the pancreas. In non-lymphoid tissues, in the absence of inflammation, the major source of constitutive expression is the macrophage lineage. Also expressed in cells from different hemopoietic cell lineages, including bone marrow cells, CD34+ stem cells, mature B- and T-cells, monocytes and granulocytes. Down-regulated in activated macrophages from inflammatory lesions of delayed-type hypersensitivity (DTH) reactions, such as in tuberculosis and in Crohn disease, whereas in Burkitt lymphoma, persists in macrophages involved in the phagocytosis of apoptotic malignant cells. |
| Function | Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation and thereby NFKB1 DNA-binding activities. In vitro, suppresses AP-1 transcription factor complex DNA-binding activities. Isoform 1: Inhibits myogenic differentiation and mediates anti-myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity resulting in reduced expression of MYOG. |
| Protein Name | Tsc22 Domain Family Protein 3Dsip-Immunoreactive PeptideProtein DipHdipDelta Sleep-Inducing Peptide ImmunoreactorGlucocorticoid-Induced Leucine Zipper ProteinGilzTsc-22-Like ProteinTsc-22-Related ProteinTsc-22r |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Isoform 1: CytoplasmNucleusLocalization Depends On Differentiation Status Of MyoblastsIn Undifferentiated MyoblastsLocalizes To The CytoplasmBut In Differentiating MyoblastLocalizes To The Nucleus |
| Alternative Antibody Names | Anti-Tsc22 Domain Family Protein 3 antibodyAnti-Dsip-Immunoreactive Peptide antibodyAnti-Protein Dip antibodyAnti-Hdip antibodyAnti-Delta Sleep-Inducing Peptide Immunoreactor antibodyAnti-Glucocorticoid-Induced Leucine Zipper Protein antibodyAnti-Gilz antibodyAnti-Tsc-22-Like Protein antibodyAnti-Tsc-22-Related Protein antibodyAnti-Tsc-22r antibodyAnti-TSC22D3 antibodyAnti-DSIPI antibodyAnti-GILZ antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TSC22D3 knockout (KO) 293T cells, using [KO Validated] TSC22D3 Rabbit polyclonal antibody (STJ11100787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89723/358204/STJ11100787_1__85282.1713122525.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TSC22D3 knockout (KO) 293T cells, using [KO Validated] TSC22D3 Rabbit polyclonal antibody (STJ11100787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
