| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRPV1 (725-839) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRPV1 |
| Gene ID: |
7442 |
| Uniprot ID: |
TRPV1_HUMAN |
| Immunogen Region: |
725-839 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 725-839 of human TRPV1 (NP_061197.4). |
| Immunogen Sequence: |
KLLQVGYTPDGKDDYRWCFR VDEVNWTTWNTNVGIINEDP GNCEGVKRTLSFSLRSSRVS GRHWKNFALVPLLREASARD RQSAQPEEVYLRQFSGSLKP EDAEVFKSPAASGEK |
| Tissue Specificity | Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). |
| Post Translational Modifications | Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phosphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PRKCE, PRKCM and probably PRKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. |
| Function | Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily V Member 1Trpv1Capsaicin ReceptorOsm-9-Like Trp Channel 1Otrpc1Vanilloid Receptor 1 |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Postsynaptic Cell MembraneMulti-Pass Membrane ProteinCell ProjectionDendritic Spine MembraneCell MembraneMostlyBut Not Exclusively Expressed In Postsynaptic Dendritic Spines |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily V Member 1 antibodyAnti-Trpv1 antibodyAnti-Capsaicin Receptor antibodyAnti-Osm-9-Like Trp Channel 1 antibodyAnti-Otrpc1 antibodyAnti-Vanilloid Receptor 1 antibodyAnti-TRPV1 antibodyAnti-VR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
