| Host: |
Mouse |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-TRPM8 (Extracellular region) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M571 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:200IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRPM8 |
| Gene ID: |
79054 |
| Uniprot ID: |
TRPM8_HUMAN |
| Immunogen Region: |
Extracellular region |
| Specificity: |
This antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 and MCF-7 cells. The antibody is also recommended for immunocytochemical labeling of cells. |
| Immunogen: |
Clone M571 was generated from TRPM8 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM8. This site is well conserved in rat and mouse TRPM8, but has low homology to other TRPM family members. |
| Function | Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration. Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 8Long Transient Receptor Potential Channel 6Ltrpc-6Ltrpc6Transient Receptor Potential P8Trp-P8 |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinMembrane RaftEndoplasmic Reticulum MembraneLocalizes To Membrane Rafts But Is Also Located In The Cell Membrane Outside Of These Regions Where Channel Response To Cold Is Enhanced Compared To Membrane RaftsLocated In The Endoplasmic Reticulum In Prostate Cancer Cells |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily M Member 8 antibodyAnti-Long Transient Receptor Potential Channel 6 antibodyAnti-Ltrpc-6 antibodyAnti-Ltrpc6 antibodyAnti-Transient Receptor Potential P8 antibodyAnti-Trp-P8 antibodyAnti-TRPM8 antibodyAnti-LTRPC6 antibodyAnti-TRPP8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
