Anti-TRPM5 antibody (STJ13100394)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from human TRPM5 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-TRPM5 for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | TRPM5 |
| Gene ID | 29850 |
| Uniprot ID | TRPM5_HUMAN |
| Immunogen | A synthetic peptide from human TRPM5 conjugated to blue carrier protein was used as the antigen. |
| Specificity | Specific for TRPM5. |
Additional Info
| Post Translational Modifications | Ser-129 phosphorylation by PKC, is essential for activating TRPM5 via the G(q) pathway. |
| Function | Monovalent cation-selective ion channel activated by intracellular Ca(2+) in a voltage- and temperature-dependent manner. Mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increase in intracellular Ca(2+), but is impermeable to it. The activation mechanism of TRPM5 involves a multistep process. TRPM5 activation involves ligand binding (i.e., tastant molecule, glucose stimulation) to Gq/G-protein coupled receptors (GPCR) and leads to the breakdown of phosphatidylinositol bisphosphate (PIP2) into diacylglycerol (DAG) and inositol trisphosphate (IP3), IP3 binds to its receptors in the endoplasmic reticulum and cause calcium release. Simultaneously with the intracellular Ca(2+) release, DAG activates the protein kinase C (PKC), which phosphorylates the TRPM5 channel. This phosphorylation combined with the bound Ca(2+), leads to a robust inward current allowing the entry of sodium ions (Na+) into the cell. This ion influx depolarizes the cell membrane, generating action potentials that propagate TRPM5 signals. Is a key player in sensing sweet, umami and bitter stimuli. Involved in insulin secretion by pancreatic beta cells. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 5Long Transient Receptor Potential Channel 5Ltrpc-5Ltrpc5Mlsn1- And Trp-Related Gene 1 Protein |
| Database Links | Reactome: R-HSA-3295583Reactome: R-HSA-9717207 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily M Member 5 antibodyAnti-Long Transient Receptor Potential Channel 5 antibodyAnti-Ltrpc-5 antibodyAnti-Ltrpc5 antibodyAnti-Mlsn1- And Trp-Related Gene 1 Protein antibodyAnti-TRPM5 antibodyAnti-LTRPC5 antibodyAnti-MTR1 antibody |
Information sourced from Uniprot.org