• STJ72181 (0. 3µg/ml) staining of HEK293 (A) and NIH3T3 (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ72181 (3. 75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-TRIM71 antibody (390-402) (STJ72181)

SKU:
STJ72181-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TRIM71 (390-402) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TRIM71
Gene ID: 131405
Uniprot ID: LIN41_HUMAN
Immunogen Region: 390-402
Accession Number: NP_001034200.1
Immunogen Sequence: RQVKAKSLYLQVE
Post Translational Modifications Autoubiquitinated.
Function E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (Probable). Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1. Specific regulator of miRNA biogenesis. Binds to miRNA MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression.
Protein Name E3 Ubiquitin-Protein Ligase Trim71
Protein Lin-41 Homolog
Ring-Type E3 Ubiquitin Transferase Trim71
Tripartite Motif-Containing Protein 71
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
P-Body
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim71 antibody
Anti-Protein Lin-41 Homolog antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim71 antibody
Anti-Tripartite Motif-Containing Protein 71 antibody
Anti-TRIM71 antibody
Anti-LIN41 antibody

Information sourced from Uniprot.org

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