Anti-TRIM69 antibody (1-341) (STJ117821)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRIM69 (1-341) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM69 |
| Gene ID | 140691 |
| Uniprot ID | TRI69_HUMAN |
| Immunogen Region | 1-341 |
| Immunogen Sequence | MEEELAIQQGQLETTLKELQ TLRNMQKEAIAAHKENKLHL QQHVSMEFLKLHQFLHSKEK DILTELREEGKALNEEMELN LSQLQEQCLLAKDMLVSIQA KTEQQNSFDFLKDITTLLHS LEQGMKVLATRELISRKLNL GQYKGPIQYMVWREMQDTLC PGLSPLTLDPKTAHPNLVLS KSQTSVWHGDIKKIMPDDPE RFDSSVAVLGSRGFTSGKWY WEVEVAKKTKWTVGVVRES |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human TRIM69 (NP_542783.2). |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation is necessary for nuclear localization. |
| Function | E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus. Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection. In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity. Also plays a role in cataract formation together with TP53. Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination. Regulates centrosome dynamics and mitotic progression by ubiquitinating STK3/MST2.leading to its redistribution to the perinuclear cytoskeleton and subsequent phosphorylation by PLK1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim69Rfp-Like Domain-Containing Protein TrimlessRing Finger Protein 36Ring-Type E3 Ubiquitin Transferase Trim69Tripartite Motif-Containing Protein 69 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusNucleus SpeckleCytoskeletonMicrotubule Organizing CenterCentrosomeAdopts A Filamentous Distribution In The Cell Cytoplasm Where It Strongly Colocalizes With Stable Microtubules |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim69 antibodyAnti-Rfp-Like Domain-Containing Protein Trimless antibodyAnti-Ring Finger Protein 36 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim69 antibodyAnti-Tripartite Motif-Containing Protein 69 antibodyAnti-TRIM69 antibodyAnti-RNF36 antibodyAnti-HSD-34 antibodyAnti-HSD34 antibody |
Information sourced from Uniprot.org