| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRIM69 (1-341) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRIM69 |
| Gene ID: |
140691 |
| Uniprot ID: |
TRI69_HUMAN |
| Immunogen Region: |
1-341 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human TRIM69 (NP_542783.2). |
| Immunogen Sequence: |
MEEELAIQQGQLETTLKELQ TLRNMQKEAIAAHKENKLHL QQHVSMEFLKLHQFLHSKEK DILTELREEGKALNEEMELN LSQLQEQCLLAKDMLVSIQA KTEQQNSFDFLKDITTLLHS LEQGMKVLATRELISRKLNL GQYKGPIQYMVWREMQDTLC PGLSPLTLDPKTAHPNLVLS KSQTSVWHGDIKKIMPDDPE RFDSSVAVLGSRGFTSGKWY WEVEVAKKTKWTVGVVRES |
| Post Translational Modifications | Phosphorylated. Phosphorylation is necessary for nuclear localization. |
| Function | E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus. Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection. In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity. Plays also a role in cataract formation together with TP53. Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim69Rfp-Like Domain-Containing Protein TrimlessRing Finger Protein 36Ring-Type E3 Ubiquitin Transferase Trim69Tripartite Motif-Containing Protein 69 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusNucleus SpeckleAdopts A Filamentous Distribution In The Cell Cytoplasm Where It Strongly Colocalizes With Stable Microtubules |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim69 antibodyAnti-Rfp-Like Domain-Containing Protein Trimless antibodyAnti-Ring Finger Protein 36 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim69 antibodyAnti-Tripartite Motif-Containing Protein 69 antibodyAnti-TRIM69 antibodyAnti-RNF36 antibodyAnti-HSD-34 antibodyAnti-HSD34 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and CD2BP2 knockout (KO) 293T cells, using [KO Validated] CD2BP2 Rabbit polyclonal antibody (STJ11100767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89704/358187/STJ11100767_1__17306.1713122501.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CD2BP2 knockout (KO) 293T cells, using [KO Validated] CD2BP2 Rabbit polyclonal antibody (STJ11100767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
