• Immunohistochemistry analysis of TRIM62 in paraffin-embedded Human colon using TRIM62 Rabbit polyclonal antibody (STJ118300) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TRIM62 Rabbit polyclonal antibody (STJ118300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRIM62 antibody (120-280) (STJ118300)

SKU:
STJ118300

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM62 (120-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM62
Gene ID: 55223
Uniprot ID: TRI62_HUMAN
Immunogen Region: 120-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human TRIM62 (NP_060677.2).
Immunogen Sequence: HEQHQVTGIDDAFDELQREL KDQLQALQDSEREHTEALQL LKRQLAETKSSTKSLRTTIG EAFERLHRLLRERQKAMLEE LEADTARTLTDIEQKVQRYS QQLRKVQEGAQILQERLAET DRHTFLAGVASLSERLKGKI HETNLTYEDFPTSKYTGPLQ Y
Post Translational Modifications Polyubiquitinated, autoubiquitinated in the presence of UBE2D2.
Function E3 ubiquitin ligase that plays a role in antifungal immunity by mediating 'Lys-27'-linked ubiquitination of CARD9 downstream of C-type lectin receptors.leading to CARD9 activation, followed by activation of NF-kappa-B and MAP kinase p38 pathways. E3 ubiquitin ligase activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.
Protein Name E3 Ubiquitin-Protein Ligase Trim62
Tripartite Motif-Containing Protein 62
Database Links Reactome: R-HSA-877300
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim62 antibody
Anti-Tripartite Motif-Containing Protein 62 antibody
Anti-TRIM62 antibody

Information sourced from Uniprot.org

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