Anti-TRIM50 antibody (312-362 aa) (STJ193829)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human TRI50 at the amino acid range 312-362
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase TRIM50 (312-362 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM50 |
| Gene ID | 135892 |
| Uniprot ID | TRI50_HUMAN |
| Immunogen | Synthesized peptide derived from the human TRI50 at the amino acid range 312-362 |
| Immunogen Region | 312-362 aa |
| Specificity | This antibody detects endogenous levels of TRI50 at Human/Mouse/Rat |
Additional Info
| Function | E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Also interacts with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction. Also promotes NLRP3 inflammasome activation by directly inducing NLRP3 oligomerization independent of its E3 ligase function. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim50Ring-Type E3 Ubiquitin Transferase Trim50Tripartite Motif-Containing Protein 50 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmLocalizes Mainly Into Discrete Cytoplasmic Punctuate Structures Heterogeneous In Size And Shape Containing Polyubiquitinated Proteins |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim50 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim50 antibodyAnti-Tripartite Motif-Containing Protein 50 antibodyAnti-TRIM50 antibodyAnti-TRIM50A antibody |
Information sourced from Uniprot.org