| Host: | Mouse |
| Applications: | WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-E3 ubiquitin-protein ligase TRIM33 is suitable for use in Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 3H7-C8-B9 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2a |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRIM33 |
| Gene ID: | 51592 |
| Uniprot ID: | TRI33_HUMAN |
| Specificity: | This antibody detects endogenous levels of TIF1 gamma/TRIM33 and does not cross-react with related proteins. |
| Immunogen: | Purified recombinant human TIF1 gamma/TRIM33 protein fragments expressed in E.coli. |
| Post Translational Modifications | Sumoylated with SUMO1. |
| Function | Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade). |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim33 Ectodermin Homolog Ret-Fused Gene 7 Protein Protein Rfg7 Ring-Type E3 Ubiquitin Transferase Trim33 Transcription Intermediary Factor 1-Gamma Tif1-Gamma Tripartite Motif-Containing Protein 33 |
| Database Links | Reactome: R-HSA-2173795 Reactome: R-HSA-9754189 |
| Cellular Localisation | Nucleus In Discrete Nuclear Dots Resembling Nuclear Bodies Localizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim33 antibody Anti-Ectodermin Homolog antibody Anti-Ret-Fused Gene 7 Protein antibody Anti-Protein Rfg7 antibody Anti-Ring-Type E3 Ubiquitin Transferase Trim33 antibody Anti-Transcription Intermediary Factor 1-Gamma antibody Anti-Tif1-Gamma antibody Anti-Tripartite Motif-Containing Protein 33 antibody Anti-TRIM33 antibody Anti-KIAA1113 antibody Anti-RFG7 antibody Anti-TIF1G antibody |
Information sourced from Uniprot.org