Anti-TRIM25 antibody (100-200) [S9MR] (STJ11102049)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal TRIM25 (100-200) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S9MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM25 |
| Gene ID | 7706 |
| Uniprot ID | TRI25_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | SPNAQVACDHCLKEAAVKTC LVCMASFCQEHLQPHFDSPA FQDHPLQPPVRDLLRRKCSQ HNRLREFFCPEHSECICHIC LVEHKTCSPASLSQASADLE A |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM25 (Q14258). |
Additional Info
| Tissue Specificity | Expressed in breast tumors (at protein level). Ubiquitous. |
| Post Translational Modifications | Auto-ISGylated. (Microbial infection) Autoubiquitinated.promoted by Epstein-Barr virus protein BPLF1. |
| Function | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks. Plays an essential role in the antiviral activity of ZAP/ZC3HAV1.an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'-linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA. Also mediates the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability. |
| Protein Name | E3 Ubiquitin/Isg15 Ligase Trim25Estrogen-Responsive Finger ProteinRing Finger Protein 147Ring-Type E3 Ubiquitin TransferaseRing-Type E3 Ubiquitin Transferase Trim25Tripartite Motif-Containing Protein 25Ubiquitin/Isg15-Conjugating Enzyme Trim25Zinc Finger Protein 147 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-168928Reactome: R-HSA-5656169Reactome: R-HSA-5689896Reactome: R-HSA-877300Reactome: R-HSA-918233Reactome: R-HSA-933541Reactome: R-HSA-933542Reactome: R-HSA-933543Reactome: R-HSA-936440Reactome: R-HSA-9692916Reactome: R-HSA-9705671Reactome: R-HSA-9833109Reactome: R-HSA-9833110Reactome: R-HSA-9833482Reactome: R-HSA-9909505 |
| Cellular Localisation | CytoplasmStress GranuleNucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin/Isg15 Ligase Trim25 antibodyAnti-Estrogen-Responsive Finger Protein antibodyAnti-Ring Finger Protein 147 antibodyAnti-Ring-Type E3 Ubiquitin Transferase antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim25 antibodyAnti-Tripartite Motif-Containing Protein 25 antibodyAnti-Ubiquitin/Isg15-Conjugating Enzyme Trim25 antibodyAnti-Zinc Finger Protein 147 antibodyAnti-TRIM25 antibodyAnti-EFP antibodyAnti-RNF147 antibodyAnti-ZNF147 antibody |
Information sourced from Uniprot.org