• Anti-Tri-Methyl-Histone H3-K79 antibody (STJ23995)
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K79 antibody (STJ23995) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K79 antibody (STJ23995) at 1:1000 dilution.
  • Western blot analysis of lysates from HeLa cells, using TriMethyl-Histone H3-K79 Rabbit polyclonal antibody (STJ23995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 300s.

Anti-Tri-Methyl-Histone H3-K79 antibody (STJ23995)

SKU:
STJ23995

£129.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tri-Methyl-Histone H3-K79 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic trimethylated peptide around K79 of human histone H3 (NP_003520.1).
Immunogen Sequence: DFKTD
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain that can bind to zinc and may bind to nucleic acid. Mutations in this gene have been identified in numerous melanoma patients and in at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance