• Western blot analysis of various lysates, using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K36 antibody (STJ23992).
  • Immunohistochemistry analysis of paraffin-embedded human lung using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human spleen using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K36 antibody (STJ23992). Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using TriMethyl-Histone H3-K36 rabbit polyclonal antibody antibody (STJ23992) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K36 antibody (STJ23992) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Chromatin immunoprecipitation analysis of extracts of Hale cells, using TriMethyl-Histone H3-K36 antibody (STJ23992) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Tri-Methyl-Histone H3-K36 antibody (32-42) (STJ23992)

SKU:
STJ23992

£129.50 - £642.50
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Host: Rabbit
Applications: WB/DB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
DB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
ChIP:5 Mu g antibody for 5 Mu g
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H3C1.H3C2.H3C3.H3C4.H3C6.H3C7.H3C8.H3C10.H3C11.H3C12
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
8290
Uniprot ID: H31_HUMAN
H31T_HUMAN
Immunogen Region: 32-42
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).
Immunogen Sequence: MARTKQTARKSTGGKAPRKQ LATKAARKSAPATGGVKKPH RYRPGTVALREIRRYQKSTE LLIRKLPFQRLVREIAQDFK TDLRFQSSAVMALQEACEAY
Tissue Specificity P68431 - NA
Q16695 - Expressed in testicular cells.
Alternative Antibody Names Anti-Histone H3.1 antibody
Anti-Histone H3/A antibody
Anti-Histone H3/B antibody
Anti-Histone H3/C antibody
Anti-Histone H3/D antibody
Anti-Histone H3/F antibody
Anti-Histone H3/H antibody
Anti-Histone H3/I antibody
Anti-Histone H3/J antibody
Anti-Histone H3/K antibody
Anti-Histone H3/L antibody
Anti-H3C1 antibody
Anti-H3FA antibody
Anti-HIST1H3A.H3C2 antibody
Anti-H3FL antibody
Anti-HIST1H3B.H3C3 antibody
Anti-H3FC antibody
Anti-HIST1H3C.H3C4 antibody
Anti-H3FB antibody
Anti-HIST1H3D.H3C6 antibody
Anti-H3FD antibody
Anti-HIST1H3E.H3C7 antibody
Anti-H3FI antibody
Anti-HIST1H3F.H3C8 antibody
Anti-H3FH antibody
Anti-HIST1H3G.H3C10 antibody
Anti-H3FK antibody
Anti-HIST1H3H.H3C11 antibody
Anti-H3FF antibody
Anti-HIST1H3I.H3C12 antibody
Anti-H3FJ antibody
Anti-HIST1H3J antibody
Anti-Histone H3.1t antibody
Anti-H3/T antibody
Anti-H3t antibody
Anti-H3/G antibody
Anti-Histone H3.4 antibody
Anti-H3-4 antibody
Anti-H3FT antibody
Anti-HIST3H3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance