• Western blot analysis of extracts of various cell lines, using TRH antibody (STJ110620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of Mouse pituitary gland tissue using TRH Rabbit polyclonal antibody (STJ110620) at a dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using TRH Rabbit polyclonal antibody (STJ110620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRH antibody (25-230) (STJ110620)

SKU:
STJ110620

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRH (25-230) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRH
Gene ID: 7200
Uniprot ID: TRH_HUMAN
Immunogen Region: 25-230
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 25-230 of human TRH (NP_009048.1).
Immunogen Sequence: QPEAAQQEAVTAAEHPGLDD FLRQVERLLFLRENIQRLQG DQGEHSASQIFQSDWLSKRQ HPGKREEEEEEGVEEEEEEE GGAVGPHKRQHPGRREDEAS WSVDVTQHKRQHPGRRSPWL AYAVPKRQHPGRRLADPKAQ RSWEEEEEEEEREEDLMPEK RQHPGKRALGGPCGPQGAYG QAGLLLGLLDDLSRSQGAEE KRQHPG
Tissue Specificity Hypothalamus. Expressed in the hair follicle epithelium (at protein level).
Function As a component of the hypothalamic-pituitary-thyroid axis, it controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth. It promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes.
Protein Name Pro-Thyrotropin-Releasing Hormone
Pro-Trh
Prothyroliberin Cleaved Into - Thyrotropin-Releasing Hormone
Trh
Protirelin
Tsh-Releasing Factor
Thyroliberin
Thyrotropin-Releasing Factor
Trf
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-416476
Cellular Localisation Secreted
Alternative Antibody Names Anti-Pro-Thyrotropin-Releasing Hormone antibody
Anti-Pro-Trh antibody
Anti-Prothyroliberin Cleaved Into - Thyrotropin-Releasing Hormone antibody
Anti-Trh antibody
Anti-Protirelin antibody
Anti-Tsh-Releasing Factor antibody
Anti-Thyroliberin antibody
Anti-Thyrotropin-Releasing Factor antibody
Anti-Trf antibody
Anti-TRH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance