• STJ70343 (1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TRC8 antibody (C-Term) (STJ70343)

SKU:
STJ70343-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TRC8 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-Recommended concentration, 3-4µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: RNF139
Gene ID: 11236
Uniprot ID: RN139_HUMAN
Immunogen Region: C-Term
Accession Number: NP_009149.2
Immunogen Sequence: HTGAAAEEFNDDT
Post Translational Modifications Autoubiquitinated. Ubiquitination is induced by sterol and leads to ist degradation via the ubiquitin-proteasome pathway.
Function E3-ubiquitin ligase.acts as a negative regulator of cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP-SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Involved in the sterol-accelerated degradation of HMGCR. This is achieved through binding of RNF139 to INSIG1 and/or INSIG2 at the ER membrane. In addition, interaction of RNF139 with AUP1 facilitates interaction of RNF139 with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase AMFR, leading to ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination.
Protein Name E3 Ubiquitin-Protein Ligase Rnf139
Ring Finger Protein 139
Ring-Type E3 Ubiquitin Transferase Rnf139
Translocation In Renal Carcinoma On Chromosome 8 Protein
Database Links Reactome: R-HSA-901032
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf139 antibody
Anti-Ring Finger Protein 139 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rnf139 antibody
Anti-Translocation In Renal Carcinoma On Chromosome 8 Protein antibody
Anti-RNF139 antibody
Anti-TRC8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance