• Immunofluorescence analysis of U2OS cells using TRAP1 Rabbit polyclonal antibody (STJ116240) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using TRAP1 Rabbit polyclonal antibody (STJ116240) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TRAP1 in paraffin-embedded Mouse kidney using TRAP1 Rabbit polyclonal antibody (STJ116240) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TRAP1 in paraffin-embedded Human colon carcinoma using TRAP1 Rabbit polyclonal antibody (STJ116240) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TRAP1 in paraffin-embedded Rat brain using TRAP1 Rabbit polyclonal antibody (STJ116240) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TRAP1 Rabbit polyclonal antibody (STJ116240) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TRAP1 antibody (445-704) (STJ116240)

SKU:
STJ116240

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRAP1 (445-704) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRAP1
Gene ID: 10131
Uniprot ID: TRAP1_HUMAN
Immunogen Region: 445-704
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 445-704 of human TRAP1 (NP_057376.2).
Immunogen Sequence: GLFMREGIVTATEQEVKEDI AKLLRYESSALPSGQLTSLS EYASRMRAGTRNIYYLCAPN RHLAEHSPYYEAMKKKDTEV LFCFEQFDELTLLHLREFDK KKLISVETDIVVDHYKEEKF EDRSPAAECLSEKETEELMA WMRNVLGSRVTNVKVTLRLD THPAMVTVLEMGAARHFLRM QQLAKTQEERAQLLQPTLEI NPRHALIKKLNQLRASEPGL AQLLVDQIYENAMIAAGLV
Tissue Specificity Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is highly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors.
Function Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.
Protein Name Heat Shock Protein 75 Kda - Mitochondrial
Hsp 75
Tnfr-Associated Protein 1
Tumor Necrosis Factor Type 1 Receptor-Associated Protein
Trap-1
Database Links Reactome: R-HSA-611105
Cellular Localisation Mitochondrion
Mitochondrion Inner Membrane
Mitochondrion Matrix
Alternative Antibody Names Anti-Heat Shock Protein 75 Kda - Mitochondrial antibody
Anti-Hsp 75 antibody
Anti-Tnfr-Associated Protein 1 antibody
Anti-Tumor Necrosis Factor Type 1 Receptor-Associated Protein antibody
Anti-Trap-1 antibody
Anti-TRAP1 antibody
Anti-HSP75 antibody

Information sourced from Uniprot.org

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