| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRAF6 (50-150) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRAF6 |
| Gene ID: |
7189 |
| Uniprot ID: |
TRAF6_HUMAN |
| Immunogen Region: |
50-150 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 50-150 of human TRAF6 (NP_004611.1). |
| Immunogen Sequence: |
MEEIQGYDVEFDPPLESKYE CPICLMALREAVQTPCGHRF CKACIIKSIRDAGHKCPVDN EILLENQLFPDNFAKREILS LMVKCPNEGCLHKMELRHLE D |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Sumoylated on Lys-124, Lys-142 and Lys-453 with SUMO1. Polyubiquitinated on Lys-124 by TRAF3IP2.after cell stimulation with IL17A. Polyubiquitinated on Lys-124.after cell stimulation with IL1B or TGFB. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. Deubiquitinated by USP10 in a TANK-dependent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage. LRRC19 induces 'Lys-63' ubiquitination. Ubiquitinated at Lys-319 by the SCF(FBXL2) complex, leading to its degradation by the proteasome. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1 on both 'Lys-48' and 'Lys-63'-linked ubiquitin chains.leading to NF-kappa-B signaling inhibition. |
| Function | E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as ECSIT, IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. Participates also in the TCR signaling by ubiquitinating LAT. |
| Protein Name | Tnf Receptor-Associated Factor 6E3 Ubiquitin-Protein Ligase Traf6Interleukin-1 Signal TransducerRing Finger Protein 85Ring-Type E3 Ubiquitin Transferase Traf6 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-166058Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-193692Reactome: R-HSA-202424Reactome: R-HSA-205043Reactome: R-HSA-209543Reactome: R-HSA-209560Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-450302Reactome: R-HSA-450321Reactome: R-HSA-5607764Reactome: R-HSA-5689880Reactome: R-HSA-5689896Reactome: R-HSA-6811558Reactome: R-HSA-9014325Reactome: R-HSA-9020702Reactome: R-HSA-933541Reactome: R-HSA-933542Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-937042Reactome: R-HSA-937072Reactome: R-HSA-9645460Reactome: R-HSA-9692916Reactome: R-HSA-9705671Reactome: R-HSA-975110Reactome: R-HSA-975138Reactome: R-HSA-975144Reactome: R-HSA-975155Reactome: R-HSA-975163Reactome: R-HSA-9758274Reactome: R-HSA-975871 |
| Cellular Localisation | CytoplasmCell CortexNucleusLipid DropletFound In The Nuclei Of Some Aggressive B-Cell Lymphoma Cell Lines As Well As In The Nuclei Of Both Resting And Activated T- And B-LymphocytesFound In Punctate Nuclear Body Protein ComplexesUbiquitination May Occur In The Cytoplasm And Sumoylation In The NucleusRsad2/Viperin Recruits It To The Lipid Droplet |
| Alternative Antibody Names | Anti-Tnf Receptor-Associated Factor 6 antibodyAnti-E3 Ubiquitin-Protein Ligase Traf6 antibodyAnti-Interleukin-1 Signal Transducer antibodyAnti-Ring Finger Protein 85 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Traf6 antibodyAnti-TRAF6 antibodyAnti-RNF85 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
