| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-TNF receptor-associated factor 2 (Internal) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRAF2 |
| Gene ID: | 7186 |
| Uniprot ID: | TRAF2_HUMAN |
| Immunogen Region: | Internal |
| Specificity: | TRAF2 Polyclonal Antibody detects endogenous levels of TRAF2 protein. |
| Immunogen: | Synthesized peptide derived from the Internal region of human TRAF2. |
| Function | Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation.this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE. |
| Protein Name | Tnf Receptor-Associated Factor 2E3 Ubiquitin-Protein Ligase Traf2Ring-Type E3 Ubiquitin Transferase Traf2Tumor Necrosis Factor Type 2 Receptor-Associated Protein 3 |
| Database Links | Reactome: R-HSA-140534Reactome: R-HSA-3371378Reactome: R-HSA-5213460Reactome: R-HSA-5218900Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5668541Reactome: R-HSA-5675482Reactome: R-HSA-5676594Reactome: R-HSA-5689880Reactome: R-HSA-69416Reactome: R-HSA-75893Reactome: R-HSA-933541Reactome: R-HSA-933542Reactome: R-HSA-9693928Reactome: R-HSA-9758274 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Tnf Receptor-Associated Factor 2 antibodyAnti-E3 Ubiquitin-Protein Ligase Traf2 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Traf2 antibodyAnti-Tumor Necrosis Factor Type 2 Receptor-Associated Protein 3 antibodyAnti-TRAF2 antibodyAnti-TRAP3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of extracts from wild type (WT) and TRAF2 knockout (KO) 293T cells, using [KO Validated] TRAF2 Rabbit polyclonal antibody (STJ11105203) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177847/369815/STJ11105203_1__55063.1713135720.jpg?c=1 "Western blot analysis of extracts from wild type (WT) and TRAF2 knockout (KO) 293T cells, using [KO Validated] TRAF2 Rabbit polyclonal antibody (STJ11105203) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
