| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRADD |
| Gene ID: | 8717 |
| Uniprot ID: | TRADD_HUMAN |
| Immunogen Region: | 199-312 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 199-312 of human TRADD (NP_003780.1). |
| Immunogen Sequence: | AQTFLFQGQPVVNRPLSLKD QQTFARSVGLKWRKVGRSLQ RGCRALRDPALDSLAYEYER EGLYEQAFQLLRRFVQAEGR RATLQRLVEALEENELTSLA EDLLGLTDPNGGLA |
| Tissue Specificity | Found in all examined tissues. |
| Post Translational Modifications | (Microbial infection) Glycosylated at Arg-235 by enteropathogenic E.coli protein NleB1, C.rodentium protein NleB and S.typhimurium proteins Ssek1 and Ssek3: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions and assembly of the oligomeric TNFRSF1A/TNFR1 complex, thereby disrupting TNF signaling. |
| Function | Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A. |
| Protein Name | Tumor Necrosis Factor Receptor Type 1-Associated Death Domain ProteinTnfr1-Associated Death Domain ProteinTnfrsf1a-Associated Via Death Domain |
| Database Links | Reactome: R-HSA-140534Reactome: R-HSA-3371378Reactome: R-HSA-5213460Reactome: R-HSA-5218900Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5675482Reactome: R-HSA-69416Reactome: R-HSA-75893Reactome: R-HSA-9693928 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonShuttles Between The Cytoplasm And The Nucleus |
| Alternative Antibody Names | Anti-Tumor Necrosis Factor Receptor Type 1-Associated Death Domain Protein antibodyAnti-Tnfr1-Associated Death Domain Protein antibodyAnti-Tnfrsf1a-Associated Via Death Domain antibodyAnti-TRADD antibody |
Information sourced from Uniprot.org
![Immunohistochemistry analysis of SAV1 in paraffin-embedded Human colon using [KO Validated] SAV1 Rabbit polyclonal antibody (STJ11101465) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/90378/359716/STJ11101465_1__88301.1713124227.jpg?c=1 "Immunohistochemistry analysis of SAV1 in paraffin-embedded Human colon using [KO Validated] SAV1 Rabbit polyclonal antibody (STJ11101465) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
