• Western blot analysis of the lysates from HUVECcells using DIL-2 antibody.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DIL-2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from Jurkat cells, using DIL-2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of Jurkat cells using TPX2 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-TPX2 antibody (301-350 aa) (STJ96077)

SKU:
STJ96077

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Targeting protein for Xklp2 (301-350 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPX2
Gene ID: 22974
Uniprot ID: TPX2_HUMAN
Immunogen Region: 301-350 aa
Specificity: TPX2 Polyclonal Antibody detects endogenous levels of TPX2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DIL-2 at the amino acid range 301-350
Function Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
Protein Name Targeting Protein For Xklp2
Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2
Dil-2
Hepatocellular Carcinoma-Associated Antigen 519
Hepatocellular Carcinoma-Associated Antigen 90
Protein Fls353
Restricted Expression Proliferation-Associated Protein 100
P100
Database Links Reactome: R-HSA-6804756
Reactome: R-HSA-8854518
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle
Spindle Pole
During Mitosis It Is Strictly Associated With The Spindle Pole And With The Mitotic Spindle
Whereas During S And G2
It Is Diffusely Distributed Throughout The Nucleus
Is Released From The Nucleus In Apoptotic Cells And Is Detected On Apoptotic Microtubules
Alternative Antibody Names Anti-Targeting Protein For Xklp2 antibody
Anti-Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2 antibody
Anti-Dil-2 antibody
Anti-Hepatocellular Carcinoma-Associated Antigen 519 antibody
Anti-Hepatocellular Carcinoma-Associated Antigen 90 antibody
Anti-Protein Fls353 antibody
Anti-Restricted Expression Proliferation-Associated Protein 100 antibody
Anti-P100 antibody
Anti-TPX2 antibody
Anti-C20orf1 antibody
Anti-C20orf2 antibody
Anti-DIL2 antibody
Anti-HCA519 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance