| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Nucleoprotein TPR (2191-2241 aa) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TPR |
| Gene ID: |
7175 |
| Uniprot ID: |
TPR_HUMAN |
| Immunogen Region: |
2191-2241 aa |
| Specificity: |
This antibody detects endogenous levels of TPR at Human |
| Immunogen: |
Synthesized peptide derived from the human TPR at the amino acid range 2191-2241 |
| Post Translational Modifications | Phosphorylated. Phosphorylation occurs on serine and threonine residues (comprised in the C-terminal region) by MAPK1/ERK2 and stabilizes the interaction between these two proteins. Proteolytically degraded after poliovirus (PV) infection.degradation is restricted to its unfolded C-terminal tail domain whereas its coiled-coil domain containing NCP- and NUP153-binding domains withstand degradation. |
| Function | Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus.in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters.associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. According to some authors, plays a limited role in the regulation of nuclear protein export. Also plays a role as a structural and functional element of the perinuclear chromatin distribution.involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases. |
| Protein Name | Nucleoprotein TprMegatorNpc-Associated Intranuclear ProteinTranslocated Promoter Region Protein |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-165054Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-168325Reactome: R-HSA-168333Reactome: R-HSA-170822Reactome: R-HSA-180746Reactome: R-HSA-180910Reactome: R-HSA-191859Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-3301854Reactome: R-HSA-3371453Reactome: R-HSA-4085377Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5578749Reactome: R-HSA-5619107Reactome: R-HSA-6784531Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9705671Reactome: R-HSA-9725370 |
| Cellular Localisation | NucleusNucleus MembranePeripheral Membrane ProteinNucleoplasmic SideNucleus EnvelopeNuclear Pore ComplexCytoplasmCytoskeletonSpindleChromosomeCentromereKinetochoreCytoplasmic SideDetected As Discrete Intranuclear Foci With Ifi204In InterphaseLocalizes To The Nucleoplasmic Side Of The Nuclear Pore Complex (Npc) Core StructureForming A Fibrous Structure Called The Nuclear BasketDetected Exclusively To The Cytoplasmic Margin Of NpcDocking To The Inner Nucleoplasmic Side Of The Npc Is Mediated Through Binding To NucleoporinsAnchored By Nup153 To The NpcThe Assembly Of The Npc Is A Stepwise Process In Which Trp-Containing Peripheral Structures Assemble After Other ComponentsIncluding P62Detected As Filaments That Emanate From The Nuclear Basket Of The Npc And Extend To The Nucleolus To Delineate A Chromatin-Free Network Extending From The Nuclear Envelope To The Perinucleolar RegionDetected In Diffuse And Discrete Spheroidal Intranuclear FociNucleocytoplasmic Shuttling Protein Imported Into The Nucleus In A Xpo1/Crm1- And Importin Alpha/Importin Beta Receptor-Dependent MannerRemains Localized To The Nuclear Membrane After Poliovirus (Pv) InfectionDuring MitosisRemains Associated With The Nuclear Envelope Until PrometaphaseAssociated With The Mitotic Spindle From Late Prometaphase Until AnaphaseReorganized During Mitosis In A Viscous And Dynamic Nuclear-Derived Spindle Matrix That Embeds The Microtubule Spindle Apparatus From Pole To Pole In A Microtubule-Independent MannerRecruited To The Reforming Nuclear Envelope During Telophase And CytokinesisDetected At Kinetochores During PrometaphaseColocalizes With Mad2l1 In The Spindle Matrix But Not At KinetochoreColocalizes With DyneinDynactinTubulin At Kinetochore During The Metaphase-Anaphase TransitionColocalizes With Dynll1 At The Mitotic Spindle |
| Alternative Antibody Names | Anti-Nucleoprotein Tpr antibodyAnti-Megator antibodyAnti-Npc-Associated Intranuclear Protein antibodyAnti-Translocated Promoter Region Protein antibodyAnti-TPR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
