Anti-TPH1 antibody (STJA0003811)
SPECIFICATIONS
ClonalityPolyclonal
HostSheep
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purification by preparative SDS-PAGE.
General Information
| Short Description | Sheep polyclonal anti-Tryptophan Hydroxylase for use in WB and IHC in Human, Rat, Bovine, Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Mouse, Non-Human Primates, Sheep and Vole samples. Datasheet included with dilution recommendations, and relat |
| Applications | WB/IHC |
| Host | Sheep |
| Reactivity | Human/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Mouse/Non-Human Primates/Sheep/Vole |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000IHC 1:1000 |
| Formulation | 100 ยตl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 ยตg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen | Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purification by preparative SDS-PAGE. |
Additional Info
| Background | Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002). Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001). |
Information sourced from Uniprot.org