| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-TPD52 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 26-28kDa band observed in Human Lymph Node and Human Kidney lysates (calculated MW of 24.3kDa according to NP_001020423.1). The observed molecular weight corresponds to earlier f |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | TPD52 |
| Gene ID: | 7163 |
| Uniprot ID: | TPD52_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_001020423.1 ; NP_001020424.1; NP_005070.1 |
| Specificity: | This antibody is expected to recognise all three reported isoforms (NP_001020423.1, NP_001020424.1and NP_005070.1). |
| Immunogen Sequence: | TEPLPEKTQES |
| Protein Name | Tumor Protein D52Protein N8 |
| Database Links | Reactome: R-HSA-432722 |
| Alternative Antibody Names | Anti-Tumor Protein D52 antibodyAnti-Protein N8 antibodyAnti-TPD52 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and TPD52 knockout (KO) HeLa cells, using [KO Validated] TPD52 Rabbit polyclonal antibody (STJ11100844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89778/358249/STJ11100844_1__21374.1713122585.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TPD52 knockout (KO) HeLa cells, using [KO Validated] TPD52 Rabbit polyclonal antibody (STJ11100844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
