• Immunohistochemistry analysis of Topo II Alpha in paraffin-embedded human cervix cancer tissue using Topo II Alpha Rabbit polyclonal antibody (STJ11105362) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Topo II Alpha in paraffin-embedded human colon carcinoma tissue using Topo II Alpha Rabbit polyclonal antibody (STJ11105362) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from HeLa cells, using Topo II Alpha Rabbit polyclonal antibody (STJ11105362) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TOP2A antibody (1431-1531) (STJ11105362)

SKU:
STJ11105362

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dna Topoisomerase 2-Alpha (1431-1531) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOP2A
Gene ID: 7153
Uniprot ID: TOP2A_HUMAN
Immunogen Region: 1431-1531
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1431-1531 of human Topo IIalpha (NP_001058.2).
Immunogen Sequence: GAKKRAAPKGTKRDPALNSG VSQKPDPAKTKNRRKRKPST SDDSDSNFEKIVSKAVTSKK SKGESDDFHMDFDSAVAPRA KSVRAKKPIKYLEESDEDDL F
Tissue Specificity Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. Also found in high-grade lymphomas, squamous cell lung tumors and seminomas.
Post Translational Modifications Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated.
Function Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation.
Protein Name Dna Topoisomerase 2-Alpha
Dna Topoisomerase Ii - Alpha Isozyme
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-4615885
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleolus
Alternative Antibody Names Anti-Dna Topoisomerase 2-Alpha antibody
Anti-Dna Topoisomerase Ii - Alpha Isozyme antibody
Anti-TOP2A antibody
Anti-TOP2 antibody

Information sourced from Uniprot.org

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