![Immunofluorescence analysis of U2OS cells using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98096/381699/STJ119224_1__25223.1713149883.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of NIH/3T3 using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98096/381700/STJ119224_2__09144.1713149884.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of C6 cells using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98096/381701/STJ119224_3__37043.1713149885.jpg?c=1 "Immunofluorescence analysis of C6 cells using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of TOM20 in paraffin-embedded rat kidney using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98096/381702/STJ119224_4__74132.1713149885.jpg?c=1 "Immunohistochemistry analysis of TOM20 in paraffin-embedded rat kidney using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of TOM20 in paraffin-embedded mouse heart using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98096/381703/STJ119224_5__83122.1713149886.jpg?c=1 "Immunohistochemistry analysis of TOM20 in paraffin-embedded mouse heart using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of TOM20 in paraffin-embedded human colon carcinoma using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98096/381704/STJ119224_6__13785.1713149887.jpg?c=1 "Immunohistochemistry analysis of TOM20 in paraffin-embedded human colon carcinoma using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and TOM20 knockout (KO) 293T cells, using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98096/381705/STJ119224_7__74661.1713149888.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TOM20 knockout (KO) 293T cells, using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of various lysates using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98096/381706/STJ119224_8__85779.1713149889.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.")
![Immunofluorescence analysis of U2OS cells using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98096/381699/STJ119224_1__25223.1713149883.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.")
| Short Description : | Rabbit polyclonal TOM20 (20-145) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TOMM20 |
| Gene ID: | 9804 |
| Uniprot ID: | TOM20_HUMAN |
| Immunogen Region: | 20-145 |
| Immunogen Sequence: | YCIYFDRKRRSDPNFKNRLR ERRKKQKLAKERAGLSKLPD LKDAEAVQKFFLEEIQLGEE LLAQGEYEKGVDHLTNAIAV CGQPQQLLQVLQQTLPPPVF QMLLTKLPTISQRIVSAQSL AEDDVE |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). |
| Post Translational Modifications | Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. |
| Function | Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases. |
| Protein Name | Mitochondrial Import Receptor Subunit Tom20 HomologMitochondrial 20 Kda Outer Membrane ProteinOuter Mitochondrial Membrane Receptor Tom20 |
| Database Links | Reactome: R-HSA-1268020Reactome: R-HSA-5205685Reactome: R-HSA-5689880 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Mitochondrial Import Receptor Subunit Tom20 Homolog antibodyAnti-Mitochondrial 20 Kda Outer Membrane Protein antibodyAnti-Outer Mitochondrial Membrane Receptor Tom20 antibodyAnti-TOMM20 antibodyAnti-KIAA0016 antibody |
Information sourced from Uniprot.org
![Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/90589/360346/STJ11101675_1__95163.1713124994.jpg?c=1 "Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)")
