• Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)
  • Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)
  • Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)
  • Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)
  • Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)
  • Immunoprecipitation analysis of 200 Mu g extracts from HeLa cells using 3 Mu g TOM20 antibody (STJ11101675). Western blot was performed from the immunoprecipitate using TOM20 antibody (STJ11101675) at a dilution of 1:1000.
  • Confocal imaging of HeLa cells using TOM20 Rabbit monoclonal antibody (STJ11101675, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of paraffin-embedded Mouse kidney using TOM20 Rabbit monoclonal antibody (STJ11101675, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of TOM20 in paraffin-embedded rat heart using TOM20 Rabbit monoclonal antibody (STJ11101675) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TOM20 in paraffin-embedded mouse kidney using TOM20 Rabbit monoclonal antibody (STJ11101675) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TOM20 in paraffin-embedded human placenta using TOM20 Rabbit monoclonal antibody (STJ11101675) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TOM20 in paraffin-embedded human colon carcinoma using TOM20 Rabbit monoclonal antibody (STJ11101675) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and TOM20 knockdown (KD) 293T, using TOM20 Rabbit monoclonal antibody (STJ11101675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates, using TOM20 Rabbit monoclonal antibody (STJ11101675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates, using TOM20 Rabbit monoclonal antibody (STJ11101675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)

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STJ11101675

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TOM20 (20-145) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
IHC-P 1:50-1:200
IF/ICC 1:100-1:500
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOMM20
Gene ID: 9804
Uniprot ID: TOM20_HUMAN
Immunogen Region: 20-145
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1).
Immunogen Sequence: YCIYFDRKRRSDPNFKNRLR ERRKKQKLAKERAGLSKLPD LKDAEAVQKFFLEEIQLGEE LLAQGEYEKGVDHLTNAIAV CGQPQQLLQVLQQTLPPPVF QMLLTKLPTISQRIVSAQSL AEDDVE
Post Translational Modifications Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Function Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Protein Name Mitochondrial Import Receptor Subunit Tom20 Homolog
Mitochondrial 20 Kda Outer Membrane Protein
Outer Mitochondrial Membrane Receptor Tom20
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-5205685
Reactome: R-HSA-5689880
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Import Receptor Subunit Tom20 Homolog antibody
Anti-Mitochondrial 20 Kda Outer Membrane Protein antibody
Anti-Outer Mitochondrial Membrane Receptor Tom20 antibody
Anti-TOMM20 antibody
Anti-KIAA0016 antibody

Information sourced from Uniprot.org

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