| Short Description : | Rabbit polyclonal TOLLIP (1-274) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TOLLIP |
| Gene ID: | 54472 |
| Uniprot ID: | TOLIP_HUMAN |
| Immunogen Region: | 1-274 |
| Immunogen Sequence: | MATTVSTQRGPVYIGELPQD FLRITPTQQQRQVQLDAQAA QQLQYGGAVGTVGRLNITVV QAKLAKNYGMTRMDPYCRLR LGYAVYETPTAHNGAKNPRW NKVIHCTVPPGVDSFYLEIF DERAFSMDDRIAWTHITIPE SLRQGKVEDKWYSLSGRQGD DKEGMINLVMSYALLPAAMV MPPQPVVLMPTVYQQGVGYV PITGMPAVCSPGMVPVALPP AAVNAQPRCSEEDLKAIQD |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human TOLLIP (NP_061882.2). |
| Post Translational Modifications | Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products. |
| Function | Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates. In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P). |
| Protein Name | Toll-Interacting Protein |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-9020702 |
| Cellular Localisation | CytoplasmEndosomeEarly EndosomeLocalized To Endo/Exosomal Vesicles |
| Alternative Antibody Names | Anti-Toll-Interacting Protein antibodyAnti-TOLLIP antibody |
Information sourced from Uniprot.org
