| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TNS3 (1-240) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TNS3 |
| Gene ID: |
64759 |
| Uniprot ID: |
TENS3_HUMAN |
| Immunogen Region: |
1-240 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human TNS3 (NP_073585.8). |
| Immunogen Sequence: |
MEEGHGLDLTYITERIIAVS FPAGCSEESYLHNLQEVTRM LKSKHGDNYLVLNLSEKRYD LTKLNPKIMDVGWPELHAPP LDKMCTICKAQESWLNSNLQ HVVVIHCRGGKGRIGVVISS YMHFTNVSASADQALDRFAM KKFYDDKVSALMQPSQKRYV QFLSGLLSGSVKMNASPLFL HFVILHGTPNFDTGGVCRPF LKLYQAMQPVYTSGIYNVGP ENPSRICIVIEPAQLLKGD |
| Tissue Specificity | Expressed in umbilical vein endothelial cells, epithelial cells, and fibroblasts cells (at protein level). Highly expressed in thyroid, kidney and placenta. Low expression in heart, skeletal muscle, spleen, liver, and lung. Expressed at higher levels in tonsil-derived mesenchymal stem cells (MSCs) than in adipose tissue-derived MSCs or bone marrow-derived MSCs. Expressed in tumor endothelial cells. Expression seems to be down-regulated in thyroid tumor tissues and in anaplastic carcinomas. |
| Post Translational Modifications | Phosphorylated on Ser/Thr and Tyr residues. Phosphorylated on Thr-323 in the C2-type tensin domain following EGF stimulation which changes its binding preference from DLC1 to the p85 regulatory subunit of the PI3K kinase complex. EGF induces tyrosine phosphorylation in a time- and dose-dependent manner. Phosphorylation of the SH2 domain enhances interaction with PEAK1. |
| Function | May act as a protein phosphatase and/or a lipid phosphatase (Probable). Involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. Increases DOCK5 guanine nucleotide exchange activity towards Rac and plays a role in osteoclast podosome organization. Enhances RHOA activation in the presence of DLC1. Required for growth factor-induced epithelial cell migration.growth factor stimulation induces TNS3 phosphorylation which changes its binding preference from DLC1 to the p85 regulatory subunit of the PI3K kinase complex, displacing PI3K inhibitor PTEN and resulting in translocation of the TNS3-p85 complex to the leading edge of migrating cells to promote RAC1 activation. Meanwhile, PTEN switches binding preference from p85 to DLC1 and the PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA. Acts as an adapter protein by bridging the association of scaffolding protein PEAK1 with integrins ITGB1, ITGB3 and ITGB5 which contributes to the promotion of cell migration. Controls tonsil-derived mesenchymal stem cell proliferation and differentiation by regulating the activity of integrin ITGB1. |
| Protein Name | Tensin-3Tensin-Like Sh2 Domain-Containing Protein 1Tumor Endothelial Marker 6 |
| Database Links | Reactome: R-HSA-8875513 |
| Cellular Localisation | Cell JunctionFocal AdhesionCell ProjectionPodosomeLocalizes To Both Focal And Fibrillar Adhesions But Is Mostly Found In Fibrillar Adhesions |
| Alternative Antibody Names | Anti-Tensin-3 antibodyAnti-Tensin-Like Sh2 Domain-Containing Protein 1 antibodyAnti-Tumor Endothelial Marker 6 antibodyAnti-TNS3 antibodyAnti-TEM6 antibodyAnti-TENS1 antibodyAnti-TPP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
