• Western blot analysis of extracts of A-549 cells, using TNS3 antibody (STJ117844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TNS3 antibody (1-240) (STJ117844)

SKU:
STJ117844

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNS3 (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNS3
Gene ID: 64759
Uniprot ID: TENS3_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human TNS3 (NP_073585.8).
Immunogen Sequence: MEEGHGLDLTYITERIIAVS FPAGCSEESYLHNLQEVTRM LKSKHGDNYLVLNLSEKRYD LTKLNPKIMDVGWPELHAPP LDKMCTICKAQESWLNSNLQ HVVVIHCRGGKGRIGVVISS YMHFTNVSASADQALDRFAM KKFYDDKVSALMQPSQKRYV QFLSGLLSGSVKMNASPLFL HFVILHGTPNFDTGGVCRPF LKLYQAMQPVYTSGIYNVGP ENPSRICIVIEPAQLLKGD
Tissue Specificity Expressed in umbilical vein endothelial cells, epithelial cells, and fibroblasts cells (at protein level). Highly expressed in thyroid, kidney and placenta. Low expression in heart, skeletal muscle, spleen, liver, and lung. Expressed at higher levels in tonsil-derived mesenchymal stem cells (MSCs) than in adipose tissue-derived MSCs or bone marrow-derived MSCs. Expressed in tumor endothelial cells. Expression seems to be down-regulated in thyroid tumor tissues and in anaplastic carcinomas.
Post Translational Modifications Phosphorylated on Ser/Thr and Tyr residues. Phosphorylated on Thr-323 in the C2-type tensin domain following EGF stimulation which changes its binding preference from DLC1 to the p85 regulatory subunit of the PI3K kinase complex. EGF induces tyrosine phosphorylation in a time- and dose-dependent manner. Phosphorylation of the SH2 domain enhances interaction with PEAK1.
Function May act as a protein phosphatase and/or a lipid phosphatase (Probable). Involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. Increases DOCK5 guanine nucleotide exchange activity towards Rac and plays a role in osteoclast podosome organization. Enhances RHOA activation in the presence of DLC1. Required for growth factor-induced epithelial cell migration.growth factor stimulation induces TNS3 phosphorylation which changes its binding preference from DLC1 to the p85 regulatory subunit of the PI3K kinase complex, displacing PI3K inhibitor PTEN and resulting in translocation of the TNS3-p85 complex to the leading edge of migrating cells to promote RAC1 activation. Meanwhile, PTEN switches binding preference from p85 to DLC1 and the PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA. Acts as an adapter protein by bridging the association of scaffolding protein PEAK1 with integrins ITGB1, ITGB3 and ITGB5 which contributes to the promotion of cell migration. Controls tonsil-derived mesenchymal stem cell proliferation and differentiation by regulating the activity of integrin ITGB1.
Protein Name Tensin-3
Tensin-Like Sh2 Domain-Containing Protein 1
Tumor Endothelial Marker 6
Database Links Reactome: R-HSA-8875513
Cellular Localisation Cell Junction
Focal Adhesion
Cell Projection
Podosome
Localizes To Both Focal And Fibrillar Adhesions But Is Mostly Found In Fibrillar Adhesions
Alternative Antibody Names Anti-Tensin-3 antibody
Anti-Tensin-Like Sh2 Domain-Containing Protein 1 antibody
Anti-Tumor Endothelial Marker 6 antibody
Anti-TNS3 antibody
Anti-TEM6 antibody
Anti-TENS1 antibody
Anti-TPP antibody

Information sourced from Uniprot.org

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