Western blot analysis of lysates from K-562 cells, using TNFAIP1 Rabbit polyclonal antibody (STJ119633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TNFAIP1 antibody (90-160) (STJ119633)

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STJ119633
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFAIP1
Gene ID: 7126
Uniprot ID: BACD2_HUMAN
Immunogen Region: 90-160
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 90-160 of human TNFAIP1 (NP_066960.1).
Immunogen Sequence: DTITLPQNRQEIKELMAEAK YYLIQGLVNMCQSALQDKKD SYQPVCNIPIITSLKEEERL IESSTKPVVKL
Post Translational Modifications Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
Protein Name Btb/Poz Domain-Containing Adapter For Cul3-Mediated Rhoa Degradation Protein 2
Hbacurd2
Btb/Poz Domain-Containing Protein Tnfaip1
Protein B12
Tumor Necrosis Factor - Alpha-Induced Protein 1 - Endothelial
Database Links Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Cellular Localisation Cytoplasm
Nucleus
Endosome
Colocalizes With Rhob In Endosomes
Alternative Antibody Names Anti-Btb/Poz Domain-Containing Adapter For Cul3-Mediated Rhoa Degradation Protein 2 antibody
Anti-Hbacurd2 antibody
Anti-Btb/Poz Domain-Containing Protein Tnfaip1 antibody
Anti-Protein B12 antibody
Anti-Tumor Necrosis Factor - Alpha-Induced Protein 1 - Endothelial antibody
Anti-TNFAIP1 antibody
Anti-BACURD2 antibody
Anti-EDP1 antibody

Information sourced from Uniprot.org

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