• Immunohistochemistry analysis of paraffin-embedded human lung using TNF-Alpha Rabbit monoclonal antibody (STJ11104248) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using TNF-Alpha Rabbit monoclonal antibody (STJ11104248) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using TNF-Alpha Rabbit monoclonal antibody (STJ11104248) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-TNF antibody (77-233) [S4248RM] (STJ11104248)

SKU:
STJ11104248

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Host: Rabbit
Applications: IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Tumor Necrosis Factor (77-233) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4248RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNF
Gene ID: 7124
Uniprot ID: TNFA_HUMAN
Immunogen Region: 77-233
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 77-233 of human TNF-alpha (NP_000585.2).
Immunogen Sequence: VRSSSRTPSDKPVAHVVANP QAEGQLQWLNRRANALLANG VELRDNQLVVPSEGLYLIYS QVLFKGQGCPSTHVLLTHTI SRIAVSYQTKVNLLSAIKSP CQRETPEGAEAKPWYEPIYL GGVFQLEKGDRLSAEINRPD YLDFAESGQVYFGIIAL
Post Translational Modifications The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated.glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Tumor necrosis factor, soluble form: The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. Promotes osteoclastogenesis and therefore mediates bone resorption. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Protein Name Tumor Necrosis Factor
Cachectin
Tnf-Alpha
Tumor Necrosis Factor Ligand Superfamily Member 2
Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form
N-Terminal Fragment
Ntf - Intracellular Domain 1
Icd1 - Intracellular Domain 2
Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form
Database Links Reactome: R-HSA-381340
Reactome: R-HSA-5357786
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-5626978
Reactome: R-HSA-5668541
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-75893
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Tumor Necrosis Factor
Membrane Form: Membrane
Soluble Form: Secreted
C-Domain 1: Secreted
C-Domain 2: Secreted
Alternative Antibody Names Anti-Tumor Necrosis Factor antibody
Anti-Cachectin antibody
Anti-Tnf-Alpha antibody
Anti-Tumor Necrosis Factor Ligand Superfamily Member 2 antibody
Anti-Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form antibody
Anti-N-Terminal Fragment antibody
Anti-Ntf - Intracellular Domain 1 antibody
Anti-Icd1 - Intracellular Domain 2 antibody
Anti-Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form antibody
Anti-TNF antibody
Anti-TNFA antibody
Anti-TNFSF2 antibody

Information sourced from Uniprot.org

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