• Immunofluorescence analysis of A431 cells using TMED3 Rabbit polyclonal antibody (STJ119715) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TMED3 in paraffin-embedded mouse lung using TMED3 Rabbit polyclonal antibody (STJ119715) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TMED3 in paraffin-embedded human colon carcinoma using TMED3 Rabbit polyclonal antibody (STJ119715) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TMED3 in paraffin-embedded rat kidney using TMED3 Rabbit polyclonal antibody (STJ119715) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TMED3 Rabbit polyclonal antibody (STJ119715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TMED3 antibody (24-180) (STJ119715)

SKU:
STJ119715

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TMED3 (24-180) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TMED3
Gene ID: 23423
Uniprot ID: TMED3_HUMAN
Immunogen Region: 24-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-180 of human TMED3 (NP_031390.1).
Immunogen Sequence: PCGAELTFELPDNAKQCFHE EVEQGVKFSLDYQVITGGHY DVDCYVEDPQGNTIYRETKK QYDSFTYRAEVKGVYQFCFS NEFSTFSHKTVYFDFQVGDE PPILPDMGNRVTALTQMESA CVTIHEALKTVIDSQTHYRL REAQDRARAEDLNSRVS
Function Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network.
Protein Name Transmembrane Emp24 Domain-Containing Protein 3
Membrane Protein P24b
P24 Family Protein Gamma-4
P24gamma4
P26
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus
Cis-Golgi Network Membrane
Golgi Stack Membrane
Endoplasmic Reticulum Membrane
Cytoplasmic Vesicle
Copi-Coated Vesicle Membrane
Probably Cycles Between Compartments Of The Early Secretatory Pathway
Alternative Antibody Names Anti-Transmembrane Emp24 Domain-Containing Protein 3 antibody
Anti-Membrane Protein P24b antibody
Anti-P24 Family Protein Gamma-4 antibody
Anti-P24gamma4 antibody
Anti-P26 antibody
Anti-TMED3 antibody
Anti-C15orf22 antibody
Anti-UNQ5357 antibody
Anti-PRO1078 antibody

Information sourced from Uniprot.org

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