• Western blot analysis of extracts of HeLa cells, using TLR5 antibody (STJ119345). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TLR5 rabbit polyclonal antibody (STJ119345) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using TLR5 rabbit polyclonal antibody (STJ119345) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-TLR5 antibody (714-858) (STJ119345)

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STJ119345

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TLR5 (714-858) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLR5
Gene ID: 7100
Uniprot ID: TLR5_HUMAN
Immunogen Region: 714-858
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 714-858 of human TLR5 (NP_003259.2).
Immunogen Sequence: HLDTQYSDQNRFNLCFEERD FVPGENRIANIQDAIWNSRK IVCLVSRHFLRDGWCLEAFS YAQGRCLSDLNSALIMVVVG SLSQYQLMKHQSIRGFVQKQ QYLRWPEDLQDVGWFLHKLS QQILKKEKEKKKDNNIPLQT VATIS
Tissue Specificity Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells.
Post Translational Modifications Phosphorylated at Ser-805 by PKD/PRKD1.phosphorylation induces the production of inflammatory cytokines. Phosphorylated at Tyr-798 upon flagellin binding.required for signaling.
Function Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.
Protein Name Toll-Like Receptor 5
Toll/Interleukin-1 Receptor-Like Protein 3
Database Links Reactome: R-HSA-168176
Reactome: R-HSA-5602680
Reactome: R-HSA-5603037
Reactome: R-HSA-975871
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Toll-Like Receptor 5 antibody
Anti-Toll/Interleukin-1 Receptor-Like Protein 3 antibody
Anti-TLR5 antibody
Anti-TIL3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance