• Immunofluorescence analysis of Jurkat cells using Human TLR3 Rabbit polyclonal antibody (STJ11105524) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Raji cells using Human TLR3 Rabbit polyclonal antibody (STJ11105524) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TLR3 using Human TLR3 Rabbit polyclonal antibody (STJ11105524) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-TLR3 antibody (24-339) (STJ11105524)

SKU:
STJ11105524

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Toll-Like Receptor 3 (24-339) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLR3
Gene ID: 7098
Uniprot ID: TLR3_HUMAN
Immunogen Region: 24-339
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 24-339 of human Human TLR3 (NP_003256.1).
Immunogen Sequence: STTKCTVSHEVADCSHLKLT QVPDDLPTNITVLNLTHNQL RRLPAANFTRYSQLTSLDVG FNTISKLEPELCQKLPMLKV LNLQHNELSQLSDKTFAFCT NLTELHLMSNSIQKIKNNPF VKQKNLITLDLSHNGLSSTK LGTQVQLENLQELLLSNNKI QALKSEELDIFANSSLKKLE LSSNQIKEFSPGCFHAIGRL FGLFLNNVQLGPSLTEKLCL ELANTSIRNLSLSNSQLST
Tissue Specificity Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
Post Translational Modifications Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. TLR3 signaling requires a proteolytic cleavage mediated by cathepsins CTSB and CTSH, the cleavage occurs between amino acids 252 and 346. The cleaved form of TLR3 is the predominant form found in endosomes. Ubiquitinated by TRIM3.leading to recognition and sorting of polyubiquitinated TLR3 by the ESCRT complexes. Ubiquitinated by ZNRF1 via 'Lys-63'-linked ubiquitin chains.leading to TLR3 lysosomal trafficking and degradation.
Function Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.
Protein Name Toll-Like Receptor 3
Cd Antigen Cd283
Database Links Reactome: R-HSA-1679131
Reactome: R-HSA-168164
Reactome: R-HSA-168927
Reactome: R-HSA-1810476
Reactome: R-HSA-5602410
Reactome: R-HSA-5602415
Reactome: R-HSA-5602566
Reactome: R-HSA-5602571
Reactome: R-HSA-9013957
Reactome: R-HSA-9013973
Reactome: R-HSA-9014325
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Early Endosome
Alternative Antibody Names Anti-Toll-Like Receptor 3 antibody
Anti-Cd Antigen Cd283 antibody
Anti-TLR3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance