![Western blot analysis of lysates from wild type (WT) and TLK2 knockout (KO) NIH/3T3 cells, using [KO Validated] TLK2 Rabbit polyclonal antibody (STJ28853) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102103/390570/STJ28853_1__99568.1713160612.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TLK2 knockout (KO) NIH/3T3 cells, using [KO Validated] TLK2 Rabbit polyclonal antibody (STJ28853) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western blot analysis of various lysates using [KO Validated] TLK2 Rabbit polyclonal antibody (STJ28853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102103/390571/STJ28853_2__04909.1713160612.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] TLK2 Rabbit polyclonal antibody (STJ28853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and TLK2 knockout (KO) NIH/3T3 cells, using [KO Validated] TLK2 Rabbit polyclonal antibody (STJ28853) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/102103/390570/STJ28853_1__99568.1713160612.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TLK2 knockout (KO) NIH/3T3 cells, using [KO Validated] TLK2 Rabbit polyclonal antibody (STJ28853) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
Anti-TLK2 antibody (1-220) (STJ28853)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TLK2 (1-220) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TLK2 |
| Gene ID | 11011 |
| Uniprot ID | TLK2_HUMAN |
| Immunogen Region | 1-220 |
| Immunogen Sequence | MMEELHSLDPRRQELLEARF TGVGVSKGPLNSESSNQSLC SVGSLSDKEVETPEKKQNDQ RNRKRKAEPYETSQGKGTPR GHKISDYFEFAGGSAPGTSP GRSVPPVARSSPQHSLSNPL PRRVEQPLYGLDGSAAKEAT EEQSALPTLMSVMLAKPRLD TEQLAQRGAGLCFTFVSAQQ NSPSSTGSGNTEHSCSSQKQ ISIQHRQTQSDLTIEKISAL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2). |
Additional Info
| Tissue Specificity | Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes. Almost undetectable in liver and lung. |
| Post Translational Modifications | Phosphorylated at Ser-750, probably by CHEK1. Autophosphorylated.phosphorylation promotes the assembly of higher order oligomers and enzymatic activity. |
| Function | Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A and ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy. |
| Protein Name | Serine/Threonine-Protein Kinase Tousled-Like 2HshpkPku-AlphaTousled-Like Kinase 2 |
| Database Links | |
| Cellular Localisation | NucleusNucleoplasmCytoplasmPerinuclear RegionCytoskeletonColocalizes With The Cytoplasmic Intermediate Filament System During The G1 Phase Of The Cell CyclePresent In The Perinuclear Region At S Phase And In The Nucleus At Late G2 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Tousled-Like 2 antibodyAnti-Hshpk antibodyAnti-Pku-Alpha antibodyAnti-Tousled-Like Kinase 2 antibodyAnti-TLK2 antibody |
Information sourced from Uniprot.org